Table 1.
Phenotypic Characterization of Immature Mutant Seedsa
| Phenotypic Characterb | ttn5-1 | ttn5-2 |
|---|---|---|
| No. of seeds examined | 85 | 11 |
| No. of endosperm nucleoli | 27 (8–71) | 27 (12–53) |
| Largest endosperm nucleolus (μm) | 24 (12–41) | 21 (9–32) |
| No. of embryo cells | 2 (1–4) | 3 (2–5) |
| Largest embryo cell (μm) | 100 (48–166) | 71 (51–99) |
a
Cleared seeds at the cotyledon stage of normal development were examined with Nomarski optics. Numbers represent averages followed by range observed.
b
Wild-type seeds have ∼150 to 300 endosperm nuclei at the heart stage of development and endosperm nucleoli that average 4 μm in diameter (Wu, 1999). Normal embryo cell diameter is 8 μm. Embryo cell number includes the suspensor.