Activation of SCA1p by Calmodulin.
Ca2+-dependent ATPase activity was determined in samples from gradient fraction 5. The vanadate-sensitive ATPase activities of vector control (triangles), SCA1p (squares), SCA1p nt52 (diamonds), and SCA1p nt85 (circles) were determined at 5 μM free [Ca2+]. The concentration of soybean calmodulin isoform 1 (SCaM-1; Lee et al., 1995) was varied from 0 to 100 nM. Data shown are the average of three independent experiments.