Figure 3.
Effect of Abiotic and Biotic Stimuli on [Ca2+]cyt Concentrations in Parsley Cells.
Bioluminescence of reconstituted aequorin was monitored in parsley cells treated with various stimuli at the times indicated (arrows). Numbers in parentheses represent amounts of phytoalexins produced by parsley cells treated as indicated relative to those produced by cells treated with 100 nM Pep-13.
(A) Addition of elicitors stimulating phytoalexin production in parsley cells. The P. sojae–derived 42-kD cell wall glycoprotein, recombinant P. syringae pv phaseolicola–derived harpin, and the polyene antibiotic amphotericin B were added at concentrations sufficient to stimulate elicitor-specific maximum phytoalexin production in parsley cells.
(B) Addition of elicitors of plant defense-associated responses incapable of stimulating phytoalexin production in parsley cells. Synthetic N-acetylchitoheptaose, a synthetic 15-mer fragment of bacterial flagellin, and purified β-megaspermin from P. megasperma were added at concentrations at least 10-fold greater than those reported to induce defense-associated responses in various plants.
(C) Cold shock was applied by adding an equal volume of ice-cold culture medium to parsley cells.