Table 1.
NiR Activity and Estimated Ks for Nitrite Transport Activity from Intact Chloroplasts
| Strains |
Ks for Nitrite Transport Activitya (μM) |
NiR Activity (mU/mg Chl)b |
|---|---|---|
| JA4 (Nar1−) | NDc | 1163 ± 381 (n = 3) |
| AU3 (Nar1+) | 4.8 ± 3.8 (n = 3) | 778 ± 29 (n = 3) |
| WT (Nar1+) | 5.5 ± 1.6 (n = 3) | 579 ± 37 (n = 3) |
| PAD41 (Nar1−) | ND | 857 ± 182 (n = 3) |
| AD7 (Nar1+) | 8 ± 4 (n = 3) | 715 ± 122 (n = 3) |
| D2 (Nar1−)d | 7 | 956 |
| AD3 (Nar1+)d | 3 | 842 |
a
Ks was determined from the progress curves (Galván et al., 1996). Statistical values represent the mean ±sd from three independent experiments.
b
mU, nmol nitrite hr −1; Chl, chlorophyll.
c
ND, not determinable under our experimental conditions.
d
Data for strains D2 and AD3 correspond to a representative experiment from two independent ones.