Figure 5.

Effect of Plant Hormones on Haustoria and SaExp mRNA Accumulation.

(A) Two-day-old seedlings (20 to 30 per well) were incubated at 30°C with water (Untreated), DMBQ (10 μM), kinetin (10 μM), or DMBQ (10 μM) plus α-naphthalene acetic acid (α-NAA; 0.1 μM); washed; and transferred to water at the times shown. After 24 hr of incubation, the seedlings were scored for haustorial development.

(B) and (C) SaExp1 and SaExp2 transcripts, respectively, were measured in 2-day-old seedlings treated for the times shown with water (Untreated), DMBQ (10 μM), kinetin (10 μM), or DMBQ (10 μM) plus α-NAA (0.1 μM).

(D) SaExp3 measured 3 or 5 hr after the same treatments described in (B) and (C).