Effect of Delay Times on SaExp1 and SaExp2 Accumulation.
(A) Two-day-old S. asiatica seedlings were exposed to DMBQ (10 μM) for 2 hr, washed, incubated for 10.5 hr in water, and reexposed to DMBQ. The photograph was taken 48 hr after the start of the experiment. Arrowheads indicate areas of induced swelling.
(B) and (C) SaExp1 and SaExp2, respectively, in seedlings treated with 10 μM DMBQ for 0, 4, and 11 hr (circles). A subset of the seedlings exposed for 4 hr was washed and placed in water for 2, 4, or 6 hr (squares) before being measured.