Table 3.
Known Nitrate-Responsive Genes: Microarray and RNA Gel Blot Data
| Microarrayb
|
RNA Gel Blotb
|
|||||
|---|---|---|---|---|---|---|
| Genea | L-20min | H-2hr | L-20min | L-2hr | H-20min | H-2hr |
| Nitrate uptake and assimilation | ||||||
| NR (NIA1) | 5.5 (1.0) | 6.3 (1.6) | 5.6 | 2.3 | 12 | 9.6 |
| NR (NIA2) | 3.5 (0.2) | 4.9 (0.5) | 8.9 | 2.4 | 16 | 31 |
| NiR | 11 (5.4) | 16 (4.3) | 140 | 40 | 130 | 130 |
| UPM1 | 11 (3.8) | 13 (2.4) | 13 | 2.2 | 17 | 17 |
| NRT1;1 (CHL1)c | 2.6 (0.5) | 1.6 (0.0) | 4.4 | 3.4 | 2.8 | 2.7 |
| NRT2 | NA | NA | 2.0 | 2.1 | 2.3 | 18 |
| Fd reduction | ||||||
| Fd | 3.6 (1.0) | 4.5 (0.5) | 5.2 | 3.2 | 3.9 | 3.9 |
| FNR chromosome IV | 6.2 (1.2) | 8.3 (0.1) | 6.4 | 7.6 | 17 | 17 |
| FNR chromosome I | 5.5 (1.6) | 9.0 (1.1) | 56 | 36 | 43 | 69 |
| Oxidative pentose phosphate pathway | ||||||
| G6PDH (clone E5) | 5.1 (1.7) | 4.4 (0.8) | 20 | 4.0 | 35 | 36 |
| G6PDH (chromosome I) | 6.4 (3.1) | 6.3 (1.6) | 18 | 3.6 | 12 | 9.5 |
| 6PGDH | 6.8 (2.4) | 7.1 (0.8) | ND | ND | ND | ND |
| Ammonium assimilation | ||||||
| Glutamine synthetase | 1.2 (0.1) | 2.5 (0.1) | ND | ND | ND | ND |
| Fd-GOGAT | NS | NS | ND | ND | ND | ND |
| NADH-GOGATc | 1.7 (0.1) | 2.8 (0.5) | ND | ND | ND | ND |
| Organic acid metabolism | ||||||
| PEP carboxylasec | 1.3 (0.1) | 1.1 (0.1) | ND | ND | ND | ND |
a
Gene symbols: NRT1;1 (CHL1), dual-affinity nitrate transporter; NRT2, high-affinity nitrate transporter; G6PDH, glucose-6-P-dehydrogenase; 6PGDH, 6-phosphogluconate dehydrogenase. Chr., chromosome.
b
L refers to inductions with 250 μM. H refers to inductions with 10 mM nitrate for the arrays and 5 mM for the RNA gel blots. Values for the arrays are averages for all clones and hybridizations for each gene. Numbers in parentheses are standard deviations. NA, not on microarray; ND, not determined; NS, on array but no signal.
c
Indicates more than one clone was present on the array so that several values were averaged to give the number in the table.