Figure 3.

Compactin treatment does not alter mGBP1 mobility. COS-1 cells were transfected with DNA encoding mGBP1 with compactin (50 μM) added to the medium 5 h after transfection and remaining until samples were prepared 42 h after transfection. Cell lysates were separated by SDS-PAGE, and endogenous H-Ras or the transfected mGBP1 was detected by immunoblotting. Lane 1 is from cells transfected with empty vector, and lanes 2 and 3 are from mGBP1-transfected cells treated either in the absence or the presence of compactin. The asterisk (*) denotes unprocessed H-Ras; the arrowhead shows fully processed, farnesylated H-Ras.