Figure 2.
FB1-Induced Lesions Resemble HR Lesions.
Transfer of 10-day-old seedlings to plates containing 1 μM FB1 or inoculation of 4-week-old leaves with P. s. maculicola 4326 avrRpt2 induced formation of macroscopic lesions. Comparisons were made by light or epifluorescence microscopic examination of stained leaves. See Methods for details.
(A) to (F) Leaves stained with lactophenol–trypan blue, revealing cell death.
(G) to (L) Leaves stained with nitroblue tetrazolium, revealing ROIs.
(M) to (O) Leaves stained with aniline blue, revealing callose deposition.
Control, MgSO4-treated leaves; FB1, FB1-treated leaves; Avirulent, P. s. maculicola ES4326 (avrRpt2)–infected leaves.