Figure 3.
Treatment of Arabidopsis with FB1 Induces Expression of Defense-Related Genes.
(A) Defense gene activation in response to increasing concentrations of FB1. RNA was isolated from seedlings treated for 4 days on agar media with various concentrations of FB1 and analyzed by RNA gel blot analysis. UBQ5 was used as a loading control.
(B) Two lower leaves of transgenic Arabidopsis harboring a PR-1 promoter::GUS reporter gene fusion were infiltrated with 10 μM FB1 solution. After 1 week, the plants were histochemically stained for GUS activity. A noninfiltrated leaf that developed lesions is shown. Plants infiltrated with 10 mM MgSO4 showed no detectable GUS activity.