Figure 6.
fbr Mutants Display Enhanced Resistance to a Virulent Bacterial Pathogen.
(A) Growth of P. s. maculicola ES4326 in wild-type Col-0 (circles), fbr1 (squares), and fbr2 (triangles) plants. Leaves were infiltrated with virulent P. s. maculicola ES4326 (closed symbols) or with the avirulent isogenic strain expressing the avrRpt2 avirulence gene (open symbols). Leaf disks were harvested at 0, 24, and 72 hr after infiltration, and bacterial counts were determined by serial dilution. The data shown are means for six leaves ±sem from a representative experiment; these experiments were repeated three times with fbr2 and five times with fbr1 with similar results. cfu, colony-forming units.
(B) Disease symptoms elicited by inoculation with P. s. maculicola ES4326. The left half of each leaf was infiltrated with bacterial suspension (104 cfu/mL), and the leaves were photographed 2 days later.