Figure 1.

RT-PCR of Wild-Type and lip1 Seedlings.

Wild-type (WT) and lip1 seedlings were grown for 7 days in continuous darkness. Shoot tissue was excised from the seedlings, and the total RNA was extracted and used to produce first-strand cDNA by using oligo(dT) primers and reverse transcriptase. The resulting cDNA was used as template in PCR reactions using two sets of primers.

(A) Primers PCCOPF and PCCOPR (see Methods), which are expected to amplify a 2016-bp fragment containing the entire pea COP1 coding region.

(B) Primers FS1 and RS1 (see Methods), which are expected to amplify a 1227-bp region within the pea COP1 open reading frame.

The PCR products produced were separated by electrophoresis in a 1% agarose gel. Numbers at left and right indicate the approximate sizes (in kilobases) of PCR products produced.