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. 2000 Oct;12(10):1927–1938. doi: 10.1105/tpc.12.10.1927

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Copyright © 2000, American Society of Plant Physiologists

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Figure 4. — Schematic Representation of COP1 Genes in Wild-Type and lip1 Peas and Predicted Pattern of Splicing.

(A) COP1 gene in wild-type peas. A 9.5-kb pea genomic DNA fragment produced using the primers PCCOPF and PCCOPR on wild-type genomic DNA was inserted into the vector pTOPO-XL, and the DNA sequence was obtained by using a directed sequencing approach. Boxes indicate regions of genomic DNA that contain the COP1 coding region; the pattern of splicing is shown below.

(B) COP1 gene in lip1 peas. A 20-kb region of overlapping DNA was obtained in PCR reactions on lip1 genomic DNA. A 9.5-kb fragment produced by use of the primers PCCOPF and PCCOR, a 3-kb fragment produced by the primers E7.F and E2.R, and an 8-kb fragment produced by the primers PCCOF and DREV2 were inserted into the vector pTOPOXL. The DNA sequence was obtained by using a directed sequencing approach, and a contig of overlapping DNA was produced. Boxes show regions of genomic DNA containing the COP1 coding region; hatched boxes indicate duplicated exon sequences. The 2.5-kb CPP region between exon 7′ and exon 1, used for subsequent promoter analysis, is also indicated by hatching. The pattern of splicing predicted from analysis of intron/exon boundaries is shown below. Exon 1 (black box) is skipped due to lack of recognized intron/exon boundary at the 5′ end.

.

Inline graphic — Schematic Representation of COP1 Genes in Wild-Type and lip1 Peas and Predicted Pattern of Splicing.

(A) COP1 gene in wild-type peas. A 9.5-kb pea genomic DNA fragment produced using the primers PCCOPF and PCCOPR on wild-type genomic DNA was inserted into the vector pTOPO-XL, and the DNA sequence was obtained by using a directed sequencing approach. Boxes indicate regions of genomic DNA that contain the COP1 coding region; the pattern of splicing is shown below.

(B) COP1 gene in lip1 peas. A 20-kb region of overlapping DNA was obtained in PCR reactions on lip1 genomic DNA. A 9.5-kb fragment produced by use of the primers PCCOPF and PCCOR, a 3-kb fragment produced by the primers E7.F and E2.R, and an 8-kb fragment produced by the primers PCCOF and DREV2 were inserted into the vector pTOPOXL. The DNA sequence was obtained by using a directed sequencing approach, and a contig of overlapping DNA was produced. Boxes show regions of genomic DNA containing the COP1 coding region; hatched boxes indicate duplicated exon sequences. The 2.5-kb CPP region between exon 7′ and exon 1, used for subsequent promoter analysis, is also indicated by hatching. The pattern of splicing predicted from analysis of intron/exon boundaries is shown below. Exon 1 (black box) is skipped due to lack of recognized intron/exon boundary at the 5′ end.

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