Table 1.
Introna | Nucleotide Positionb |
5′ Donor | 3′ Acceptorc |
---|---|---|---|
1 | 307–648 | AAG GTTGGCTTCG | ACATTTGTAG CTA |
2 | 727–2938 | AAG GTAGGTGATA | CTTGAAACAG GGC |
3 | 3094–3374 | AAG GTGTGCTTGA | TCATCTGTAG GTG |
4 | 3732–4745 | CAG GTTATCTTAG | CTTGGTCCAG TTC |
5 | 4905–5010 | CAG GTACTACTAT | ATTGAAACAG CCG |
6 | 5076–6946 | AAG GTGAGTTATT | TGTATTGCAG CAT |
7 | 7026–7785 | GCG GTAAGATGGA | TGCTTTTCAG GTC |
8 | 7943–8057 | AAG GTAATTGTGA | CCTTTGGCAG AGT |
9 | 8166–8482 | AAG GTATTCTATA | CTTTGAGCAG GTC |
10 | 8587–8884 | GCA GTATGTTCCT | ATTTTTACAG GTT |
11 | 9074–9220 | CCA GTACGTAATC | ATGTGTACAG GTT |
12 | 9331–9411 | AAG GTTTGTGATC | TCCTTTGCAG GAA |
7′ | AAACCTCACC ATGd | ||
Consensus | CorGAG GTAAGT | TTTTTTGCAG G |
Introns were identified by comparing the sequence of the COP1 cDNA identified using RT-PCR with COP1 genomic DNA.
Nucleotide position shown is relative to start codon.
Splice sites underlined were not identified as boundary elements by NetPlantGene intron prediction software.
Sequence of 3′ boundary of intron 7′ from the COP1 gene in lip1 peas.