Figure 4.

UV-B–Induced Steady State Transcript Amounts and Protein Accumulation of LUC, CHS, and Ubiquitin in Transformed Parsley Cell Lines.

(A) RNA gel blot analyses of transcript amounts of LUC, CHS, and ubiquitin (UBI) in response to exposure to UV-B light pulses. A stably transformed parsley line harboring a CHS promoter–LUC reporter fusion was kept in darkness (D) or irradiated with a UV-B light pulse through a 305-nm cutoff filter and kept in the dark until harvest after 2 or 6 hr. RNA gel blot analysis was performed as in Figure 3, except that one additional probe for LUC was used.

(B) Immunodetection of CHS and LUC proteins in response to UV-B light pulses. Cells were treated as described in (A), and 15 μg of protein per lane was resolved by SDS-PAGE. CHS and LUC were detected on gel blots by using specific antibodies.