Table 4.
Additive Root Hair Phenotypes of Double Mutants
| Phenotypeb
|
|||||||
|---|---|---|---|---|---|---|---|
| Genotypea | % Hairs ⩾40 μm Longc |
Length of Hairs ⩾40 μm Long (% Wild-Type Length)d |
% Multiple Hairsc,e | Other Featuresf | |||
| Class 1g | |||||||
| cow1-2 bst1 | 96 sd ± 6 (5) | 10 sd ± 0.6 (5) | 23 sd ± 2 (5) | Sometimes crooked or curled | |||
| cow1-2 cen2-1 | 90 sd ± 9 (6) | 11.2 sd ± 2.1 (5) | 34 sd ± 5 (6) | Often crooked or curled | |||
| cen3-2 cow1-1 | 100 (5) | 15.8 sd ± 2.9 (5) | 8 sd ± 3 (5) | Some bulbous bases; sometimes crooked or curled; wide | |||
| rhd3-7 cow1-1 | 100 (5) | 13.6 sd ± 2.2 (5) | 8 sd ± 6 (5) | Wavy; crooked; wide | |||
| tip1-1 cen3-2 | 100 (5) | 13.4 sd ± 1.8 (5) | 27 sd ± 9 (5) | Some bulbous bases; sometimes curled | |||
| tip1-2 shv1-4 | 9 sd ± 3 (5) | 14.4 sd ± 8.7 (5) | ND | Straight; wide; length variable | |||
| Class 2 | |||||||
| cen1-1 bst1 | 100 (5) | 9.2 sd ± 1.5 (5) | 10 sd ± 3 (5) | Sometimes curled; some bulbous bases | |||
| cen3-1 cen1-1 | 51 sd ± 11 (5) | 9 sd ± 0.8 (5) | 18 sd ± 9 (5) | Sometimes curled | |||
| cen2-1 cen3-2 | 50 sd ± 2 (5) | 8.0 sd ± 2.9 (5) | 36 sd ± 4 (5) | Sometimes curled; occasional bulbous bases | |||
| tip1-1 bst1 | 71 sd ± 11 (5) | 8.3 sd ± 2.6 (5) | 44 sd ± 2 (5) | Bulbous bases; hair diameter varies | |||
| scn1-1 bst1 | 19 sd ± 5 (5) | 6.7 sd ± 0.9 (5) | 0 (5) | Most hairs bloated, formless balloons | |||
| scn1-1 cen1-1 | 13 sd ± 4 (5) | 7.0 sd ± 0.5 (5) | 100 (5) | Multiple hairs almost merged into one large balloon | |||
| cen2-1 scn1-1 | 12 sd ± 4 (5) | 5.2 sd ± 0.2 (5) | 100 (5) | Multiple hairs almost merged into one large balloon | |||
| scn1-1 cen3-2 | 100 (5) | 7.3 sd ± 1.8 (5) | 100 |
Some hairs have side branches or branch at tip; wide; sometimes curled |
|||
| scn1-1 cow1-1 | 100 (5) | 10.3 sd ± 0.6 (5) | 100 (5) | Wide; crooked; curled | |||
| scn1-1 shv3 | 1 sd ± 1 (5) | ND | ND | Single, large swelling | |||
| shv2-1 bst1 | 5 sd ± 1 (5) | 8.2 sd ± 2.5 (5) | ND | Often crooked | |||
| shv2-1 cow1-2 | 19 sd ± 4 (5) | 9.5 sd ± 4.2 (5) | 29 sd ± 6 (5) | Crooked or curled; sometimes wide toward the tip | |||
| tip1-1 scn1-1 | 37 sd ± 5 (5) | 13.4 sd ± 1.9 (5) | 98 sd ± 3 (5) | Some hairs bloated, others spiky | |||
| Class 3 | |||||||
| bst1 cen2-1 | 99 sd ± 1 (5) | 20.6 sd± +>± (5) | 5 sd ± 3 (6) | Wide; often curled; sometimes crooked | |||
| cen2-1 cen1-2 | 100 (5) | 15.0 sd± −>′ (5) | 0 (5) | Often curled | |||
| cen2-1 rhd3-7 | 100 (6) | 22.2 sd± + (6) | 5 sd ± 4 (6) | Wavy; crooked; not curled | |||
| rhd3-1 bst1 | 100 (6) | 13.8 sd± +>° (5) | 3 sd ± 5 (6) | Wavy; crooked; hair length variable | |||
| rhd3-1 cen1-2 | 100 (5) | 10.4 sd± +>° (5) | 0 (5) | Wavy; sometimes curled | |||
| rhd3-1 cen3-2 | 100 (5) | 16.1 sd± ″>× (5) | 3 sd ± 1 (5) | Some bulbous bases, sometimes curled; not wavy | |||
| rhd3-1 tip1-2 | 100 (5) | 14.9 sd± −>+ (5) | 44 sd ± 10 (5) | Wide; occasionally crooked; not wavy | |||
Single mutant phenotypes for comparison are listed in Table 1.
Features in boldface are synergistic. Features in italic are less severe than one would predict from combining the parental phenotypes (although some influence of both parental mutations can still be seen). Features underscored are much closer to those of one parent than one would predict from combining the parental phenotypes. Numbers within parentheses are number of roots. ND, not determined.
Fifty mature hairs were assessed on each root.
Ten mature hairs were measured on each root. Wild-type length was ∼900 μm.
Multiple hairs at a single site of hair formation.
Between 50 to 500 roots of each genotype were examined.
rhd1 scn1-1, rhd1 cen1-2, rhd1 rhd2-3, rhd1 cen2-1, rhd1 shv3, rhd1 cen3-2, rhd1 tip1-2, rhd1 shv2-1, rhd1 bst1, rhd1 rhd3-7, and rhd1 shv1-4 all had additive phenotypes with large swellings characteristic of rhd1 at the base of hairs that were similar to the hairs on the other parental line.