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Calcium dependence of the normalized open probability of RyR channels in the absence and presence of protamine. Channel activity was recorded in the presence of different calcium concentrations on the cytosolic side of the RyR. Individual points represent means ± SD for control measurements (n = 6; ▪) and for the open probability in the presence of 40 μg/ml protamine (n = 3; ○). If vertical error bars are not visible, the SD of the open probability is smaller than the symbol.
