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Fig. 8. Activity assays with truncated L.casei HprK/P. (A) HPr kinase assay. HPr at 12 µM was incubated for 10 min at 37°C with 400 nM HprK/P and increasing concentrations of Pi (0, 2, 5 and 20 mM) in the presence of 5 mM MgCl2, 10 mM FBP and 1 mM ATP. (B) P-Ser-HPr phosphatase assay. P-Ser-HPr at 12 µM was incubated for 5 min at 37°C with 88 nM HprK/P and increasing concentrations of Pi (0, 0.2, 1 and 3 mM) in the presence of 5 mM MgCl2.
