Table I. Expression of the ynaJ′–lacZ fusion in QB7144 and its derivatives carrying various hprK alleles.
| Strain | hprK allele | β-galactosidase activitya |
|---|---|---|
| QB7144 | hprK+ B.subtilis | 568 |
| TG100 | hprK+ L.casei | 562 |
| M181A | G58S G270R | 7 |
| TG101 | V267F | 5 |
| TG102 | D34V L152M H223Y | 247 |
| TG103 | N272I | 253 |
| TG104 | G160S | 106 |
| TG200 | G270E | 6 |
| TG201 | E163K | 17 |
| TG203 | V267F and ccpA | 582 |
| TG113 | hprK+ B.subtilis | 563 |
| TG114 | V265F B.subtilis | 35 |
aβ-galactosidase activity was measured in cells grown in CSK medium containing 0.2% xylose. Presented are the mean values of three independent experiments, which exhibited standard deviations of less than ±10%.