Table III. Growth of various B.subtilis strains in C mineral medium supplemented with different carbon sources.
Strain and relevant genotypea | PTS sugars |
Non-PTS sugars |
||||||
---|---|---|---|---|---|---|---|---|
Glucose | Fructose | Mannitol | Maltose | Glycerol | Gluconate | Ribose | Glucitol | |
TG100 | + | + | + | + | + | + | + | + |
hprK+ | ||||||||
TG101 | – | – | – | – | + | – | – | – |
hprKV267F | ||||||||
TG202 | + | + | + | + | + | + | + | + |
ccpA | ||||||||
TG203 | +/– | – | – | +/– | + | + | + | + |
ccpA hprKV267F | ||||||||
TG107 | + | + | + | + | + | + | + | + |
ptsH1 | ||||||||
TG108 | + | + | + | + | + | + | – | + |
ptsH1 hprKV267F | ||||||||
TG122 | + | + | + | + | + | – | + | + |
ptsH1 crh hprKV267F |
aStrains were grown on solid C minimal medium supplemented with the indicated carbon sources (0.2% w/v) at 37°C and growth was checked after 2 days. (+), growth with a colony size >3 mm; (–), no growth at all or formation of tiny translucent colonies <0.5 mm in diameter; (+/–), intermediate growth with a colony size between 1 and 2 mm. Glutamate (0.25 mM) was added to the minimal medium to allow growth of ccpA mutants (Faires et al., 1999).