| oligo1 ACTCCATGGCAGACAGCGTG |
Construction of |
| oligo2 TACGGTACCAATGAACTTCCAG |
pHPRKErm |
| oligo3 GTCGAGCTCGGAAAAGCTAGC |
|
| oligo4 TTGCCATGGTATGTTCCTCC |
|
| oligo5 GAATGGTACCTCTAGCACAAA |
|
| oligo6 CGGGAATTCAAGCTTAATTG |
|
| oligo7 GCGGAATTCATTGAAGACGG |
|
| oligo8 CGCAAGCTTCCAGAACGAAA |
|
| |
|
| HPrKBam GTGGGATCCATGGCAGACAGC |
Cloning of L.casei hprK in pQE30 |
| HPrKBs1 CATACCATGGCAAAGGTTCG |
Cloning of B.subtilis hprK in pHPRKErm |
| HPrKBs2 GAAGGATCCATGGCAAAGGTTCG |
Cloning of B.subtilis |
| HPrKBs3 AACGGTACCTCCTATTCTTCTTG |
hprK in pQE30 |
| V265F2 GACGATTCCCTTCCGCCCAGGCC |
Mutagenesis of B.subtilis hprK
|
