Fig. 3. Decrease in [Ca²⁺]_m during mitochondria-driven [Ca²⁺]_c waves. Measurement of [Ca²⁺]_m was carried out using a low affinity Ca²⁺ tracer, rhod2FF, simultaneously with measurement of [Ca²⁺]_c using fluo3FF. [Ca²⁺]_c waves were evoked as described in Figure 1. The graph shows traces of [Ca²⁺]_m and [Ca²⁺]_c calculated for three subregions of the cell chosen to be in line with the apparent direction of wave propagation.