Fig. 8. Mitochondria-driven waves of caspase activation in intact myotubes. Simultaneous imaging of ΔΨ_m and fluorescent caspase cleavage products in single intact adherent myotubes treated as described in Figure 7A. After the Ca²⁺ signal-induced depolarization wave or the uncoupler (FCCP 5 µM + oligomycin 10 µg/ml)-induced depolarization was completed, the buffer was replaced with RPMI containing 5 (upper and middle) or 10 µM (lower) cell-permeable fluorogenic caspase substrate (+ PhiPhiLux). Overlaid images of F_TMRE (red) and F_PhiPhiLux (blue) are shown.