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Immunolocalization of AtVPS45 on ultrathin cryosections from Arabidopsis roots. (A and B) Sections were incubated with AtVPS45 antibodies followed by biotinylated goat anti-rabbit secondary antibodies and detected with the use of streptavidin conjugated to 10-nm gold particles. (C) Control section probed as for A and B except for the use of preimmune serum in place of the AtVPS45 antibodies. G, Golgi. Bar, 0.1 μm.
