Figure 9.

Summary of results. The t-SNAREs AtTLG2a and AtTLG2b reside on distinct domains of the TGN, where each is found in separate complexes with the Sec1p family member AtVPS45. Unlike the homologous yeast Sec1p family member Vps45p, AtVPS45 is not found on the PVC, nor does it interact with the PVC t-SNAREs AtPEP12 and AtVAM3. Although the highly homologous SNAREs AtVTI1a and AtVTI1b are each found on the TGN, only AtVTI1b is found in AtTLG2a-AtVPS45 and AtTLG2b-AtVPS45 complexes. AtVTI1a has been shown previously to be localized also to the PVC and to be found in complexes with the PVC t-SNAREs AtPEP12 and AtVAM3 (Sanderfoot et al., 1999).