Table 1.
Quantitation of immunogold electron microscopy double labeling
| Separate | Together | Total | |
|---|---|---|---|
| AtVPS45 + AtELP | 23 | 27 | 50 |
| AtVPS45 + AtPEP12 | 28 | 2 | 30 |
| AtVPS45 + AtTLG2a | 27 | 27 | 54 |
| AtVPS45 + AtTLG2b | 13 | 13 | 26 |
| AtTLG2a + AtTLG2b | 22 | 2 | 24 |
| AtTLG2a + AtELP | 22 | 9 | 31 |
| AtTLG2b + AtELP | 3 | 16 | 19 |
| AtVPS45 + AtVTI1a | 11 | 21 | 32 |
| AtVPS45 + AtVTI1b | 23 | 10 | 33 |
For each combination of proteins shown, the number of Golgi stacks in which colocalization was seen (Together; defined as within 80 nm), with antibodies against epitope-tagged or endogenous proteins, and in which the labeling was seen over different membranes or membrane domains (Separate), is indicated. The total number of Golgi stacks examined for each combination is indicated in the Total column.