Fig. 2. Phosphorylation of ERK, JNK and p38 after phorbol-ester treatment of LB-17 mouse T-lymphoma cells. LB-17 cells (5 × 10⁶ per well) were induced with TPA (40 ng/ml) for 10 min or treated with an equivalent volume of DMSO (solvent) as control, harvested and subjected to SDS–PAGE followed by western blotting with phospho specific and pan-antibodies against ERK (A), JNK (B) and p38 (C).