Fig. 2. Expression of CatL in resting and IFNγ-stimulated BM macrophages from WT, Ii–/–, p31 and p41 mice. Immunoblot analysis performed on 15 µg of cell lysate obtained from day 6 BM macrophages cultured for 48 h in the absence (–) or presence (+) of IFNγ, and analyzed by SDS–PAGE on a 15% gel under reducing conditions. An antiserum recognizing the proform and the mature forms of CatL (A), or an antiserum raised against CatD (B) was used. p31 and p41 mice selectively express one Ii isoform.