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Fig. 4. Siah-1 promotes OBF-1 degradation by the proteasome. L929 cells were transfected with Myc-tagged OBF-1 and HA-tagged Siah-1 expression vectors, as indicated. After treatment for 12 h with the proteasome inhibitor MG262, or with H2O as control, extracts were prepared and proteins detected by western blotting.
