Table 2.

Yeast genes encoding organelle-specific proteins with a single TMD

Organelle	Corresponding genes
ER	ALG5, CNE1, CPR4, CUE1, CWH41, DPM1, FKB2, GPI12, GPI3, GPI8, HRD3, IRE1, MNS1, OST1, OST4, PBN1, SBH1, SCS2, SEC12, SEC20, SEC66, SED4, SPC3, SRP102, SSS1, TSC10, UBC6, UFE1, WBP1
Golgi, cis	ANP1, GDA1, HOC1, MNN5, MNN2, MNN9, MNN10, MNN11, OCHI, VAN1, SED5
Golgi, medial	MNN1, MNN4, MNN6, MNTI, YGL257c, YIL014w, YOR320c, YLR361c
TGN	KEX1, KEX2, STE13, TLG1, TLG2, VPS10
Plasma membrane	AXL2, FET3, FUS1, HKRI, MID2, MSB2, MTL1, PMP1, SLG1, SSO1, WSC2, WSC3

Locations are as described in YPD (Hodges et al., 1999), except YOR320c and YLR361c (C.A.R. Wiggins and S. Munro, unpublished). For some enzymes in the medial Golgi, location was inferred because the protein is required for a biochemical modification that competes with, or is immediately preceded by, that of a known medial enzyme. Such proteins are likely to be later than the cis Golgi, but it is possible that some are also found to some extent in the TGN, as has been suggested for Mnn1p itself (Graham et al., 1994).