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Mitochondrial repair activity is highly sensitive to EDTA. EDTA sensitivity of repair for both nuclear and mitochondrial lysates was assessed on the GT5′ heteroduplex. Data is from three separate experiments. A concentration of EDTA much lower than is often used for isolating mitochondria is shown to have a severely inhibitory effect on repair activity. HeLa ± EDTA (200 µM), P = 0.005; Percoll, ± EDTA, P = 0.0035.
