Table 1. Mitochondrial MMR requires ATP.
| Lysate ± components | % Repair heteroduplex template | |
|---|---|---|
| GT5′ | GT3′ | |
| HeLa | 67.8 ± 5 (n = 7) | 62.1 ± 3.8 (n = 7) |
| HeLa + SBa | 74.9 (n = 2) | ND |
| HeLa – ATPb | –8.4 ± 10.1 (n = 3) | –4.1 ± 11.3 (n = 3) |
| HeLa + sulfurylase +ATPc | 34.9 ± 3.8 (n = 6) | ND |
| Percoll | 28.7 ± 2.4 (n = 8) | 26.1 ± 3.1 (n = 8) |
| Percoll + SBa | 23.1 (n = 2) | ND |
| Percoll – ATPb | –10.2 ± 14.1 (n = 3) | 0.85 ± 2.9 (n = 3) |
| Percoll + sulfurylase + ATPc | 29.7 ± 4.1 (n = 3) | ND |
ND, not determined.
aSB indicates that activity was assessed after the addition of sulfurylase buffer without the addition of the enzyme.
bConditions identical to those in footnote a, except for the addition of ATP sulfurylase, and the non-inclusion of ATP in reaction buffers.
cConditions identical to those in footnote b, but following sulfurylase treatment the ATP is replenished to 4 mM.