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Establishment of Schwann cell/neuronal cocultures. (A) Schwann cells were seeded onto neuronal cultures. (B) Day 3 after seeding. (C) Day 7 after seeding. (D) Three days after induction of myelin synthesis with ascorbate. (E) Magnified view of four Schwann cells that are actively forming myelin (arrows indicate Schwann cell bodies). Images were obtained with phase contrast microscopy. Bars,100 μm (A-D) and 10 μm (E).
