Table 1.

Genes induced during myelin synthesis using mRNA differential display PCR from premyelinating (3 days after induction) and myelinating (5 days after induction) Schwann cell/neuronal co-cultures

Identified Genes	Primersa	Cocultures (fold change)b	Cocultures + RU-486 (fold change)b	Schwann cells + P (fold change)c	DRG Neurons + P (fold change)c
3-β-hydroxysteroid dehydrogenase	HT11C/HAP-0	30 ± 2.5	25 ± 3.0	Not detectedd	Not detectedd
Calcineurin A	HT11C/HAP-0	3.3 ± 0.3	2.5 ± 0.7	0.8 ± 0.3	1.4 ± 0.2
Rat placenta cDNA clone no. RPLCT57	HT11A/HAP-4	Undetermined	Undetermined	Undetermined	Undetermined

^a Designated primers were provided by the RNAimage Differential Display System (GenHunter Corp.). 

^b The fold induction of genes were relative to cocultures at 3 days before induction. All genes were verified using RT-PCR of cDNA from premyelinating and myelinating cocultures ± RU-486. (See MATERIALS AND METHODS section). 

^c The fold induction of genes was relative to Schwann cells or DRG neurons without the addition of progesterone. 

^d 3β-HSD was undetectable using RT-PCR for Schwann cells or DRG neurons with and without the addition of progesterone.