Table 2.
Genes induced by progesterone using mRNA differential display PCR from DRG neuronal cultures with and without the addition of progesterone (100 nM)
| Identified genes | Primersa | Cocultures (Fold Change)b | Cocultures + RU-486 (Fold Change)b | Schwann cells + P (Fold Change)c | DRG Neurons + P (Fold Change) |
|---|---|---|---|---|---|
| Rap 1 b | HT11C/HAP-5 | 5.0 ± 1.0 | 1.3 ± 0.1 | 0.7 ± 0.2 | Inducedd |
| PRPP synthase-associated protein-39 kDa | HT11C/HAP-3 | 9.5 ± 1.5 | 1.1 ± 0.5 | 1.3 ± 0.4 | Inducedd |
| Nonmuscle myosin light chain | HT11C/HAP-2 | 0.72 ± 0.2 | 1.3 ± 0.2 | 0.9 ± 0.3 | 0.7 ± 0.4 |
| Rat ovary cDNA clone no. ROVAA30 | HT11G/HAP-4 | Undetermined | Undetermined | Undetermined | Undetermined |
a
Designated primers were provided by the RNAimage Differential Display System (GenHunter Corp.).
b
The fold induction of genes was relative to cocultures at 3 days before induction. All genes were verified using RT-PCR of cDNA from premyelinating and myelinating co-cultures ± RU-486. (See MATERIALS AND METHODS section).
c
The fold induction of genes was relative to Schwann cells or DRG neurons without the addition of progesterone.
d
The genes were undetectable using RT-PCR in DRG neurons without the addition of progesterone.