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. 2003 Feb 1;31(3):935–943. doi: 10.1093/nar/gkg174

Figure 4.

Figure 4

zCRY1a facilitates nuclear accumulation of zCLOCK1 and zBMAL3. (A) Sub-cellular localization of one zebrafish clock protein transiently transfected in NIH3T3 cells with or without the other protein. Representative examples of fluorescent cells. HA-tagged zCRYs and FLAG-tagged zCLOCK1 or zBMAL3 were stained respectively with a combination of anti-HA antibody and FITC-conjugated secondary antibody (green) or anti-FLAG antibody and Cy3-conjugated secondary antibody (red). Nuclei were made visible with DAPI (blue). (B) Quantitative analysis of the sub-cellular localization of each zCRY in the absence or presence of co- expressed zCLOCK1 or zBMAL3. In each experiment 150–250 cells were evaluated for nuclear (N>C, black bars), nuclear–cytoplasmic (N=C, gray bars) and cytoplasmic (N<C, white bars) fluorescence. Values shown are representative of three independent experiments. (C) Quantitative analysis of the sub-cellular localizations of zCLOCK1 and zBMAL3 in the absence or presence of each co-expressed zCRY, as described in (B).