Table 3. Detection of VirE3 protein translocation from Agrobacterium into yeast^a.

Agrobacterium strainb	No. yeast coloniesc		Output yeastd 10–4	Output Agroe 10–6	Excision efficiencyf
	Not diluted	10–2 Dilution
1100 (Cre)	6	0	201	94	3 × 10–6
1100 (NLS::Cre::VirFΔ42N)	Full	115	326	76	3 × 10–3
1100 (Cre::VirE2)	192	1	250	31	8 × 10–5
1100 (NLS::Cre::VirE3)	295	0	275	85	1 × 10–4

^aThe recipient yeast strain contains a floxed URA3 gene at chromosome V. Agrobacterium and yeast were co-cultivated for 6 days at 22°C and plated on medium containing 0.1% FOA.

^bAgrobacterium strain LBA1100 (wild type, disarmed pTi) carried a non-mobilisable plasmid expressing Cre alone (negative control), NLS::Cre::VirFΔ42N, Cre::VirE2 or NLS::Cre::VirE3, respectively.

^cAfter co-cultivation 100 µl of cells were plated (not diluted and 10^–2) on medium containing 0.1% FOA. The number of yeast Ura^– colonies was determined after 4 days at 30°C. Full, too many colonies to count.

^d,eThe output number of yeast and Agrobacterium cells was determined on LC and MY medium, respectively.

^fThe Cre-excision efficiency is determined by the number of yeast colonies on medium containing FOA per number of surviving yeast colonies (output yeast).