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. 2003 Feb 1;31(3):922–934. doi: 10.1093/nar/gkg182

Figure 2.

Figure 2

FISH mapping of YAC clones carrying human alphoid DNA. The satellite-positive isolates mapped at or near the human centromere. Some satellite sequences cross-hybridized with more than one centromere. (A) YAC clone hybridized to centromere of chromosome 15 (no. 26 in Table 1). (B) YAC clone hybridized to centromeres of chromosomes 13 and 21 (no. 13 in Table 1). (C) YAC clone hybridized to centromere of chromosome 11 (no. 2 in Table 1) (multiple signals are due to cross-hybridization with other centromeres). (D) YAC clone hybridized (nos 5 and 11 in Table 1) to the centromere of chromosome 22. (E) Two-color FISH was carried out with probes from BAC 11 (Spectrum Orange) and BAC 5 (Spectrum Green). Both probes hybridized to the centromere of chromosome 22. (F) Fiber-FISH using the same probes (bottom) as in (E).