Table 2. Distribution of pJ-alpha and CENP-B boxes in different alphoid DNA arrays.
Alphoid DNA construct | Chromosome origin | Size of alphoid DNA insert (kb) | HOR | Frequency of pJ-alpha boxes | Frequency of CENP-B boxes |
---|---|---|---|---|---|
YAC/BAC5 | 22 | 140 | Unregular | 17/54 | 11/54 |
YAC/BAC11 | 22 | 140 | 2.1 kb, 2.8 kb | 59/105 | 0/105 |
YAC/BAC3 | ΔYq74 | 140 | 5.7 kb | 372/400 | 0/400 |
A satellite unit is assumed to be box-positive if the number of mismatches between pJ-alpha/CENP-B box in a unit and pJ-alpha and CENP-B consensus sequences (YTTCGTTGGAARCGGGA and CTAYGGTGRAAAAGGAA) is ≤3.