Table 3. Efficiency of HAC formation by different alphoid DNA arrays.
Alphoid DNA construct | Chromosome origin | Size of array (kb) | Type of array | Frequency of HAC-positive colonies | % of cells with HAC in the colony |
---|---|---|---|---|---|
YAC/BAC11 | 22 | 140 | Aa | 2/16 | 20; 80b |
YAC/BAC3 | ΔYq74 | 140 | Aa | 0/28 | |
Pac_7c5c | 21 | 70 | A+Ba | 8/10 | 80d |
aArrays with HOR structures.
bEach individual colony contains either 20 or 80% of cells with HAC.
cPac_7c5 construct was used as a positive control (11).
dThe eight positive colonies with ≥80% of cells with HAC. Pac_7c5 HACs were not validated.