Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Feb 1;31(3):981–987. doi: 10.1093/nar/gkg184

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003 Oxford University Press

PMC Copyright notice

Suppression of the expression of the endogenous H-ras gene. (A) Ten sites (sites 1–10) were chosen as targets for synthetic siRNAs, as detailed in the text. For the preparation of diced siRNAs, long dsRNAs corresponding to the 3′ region of the H-ras gene (nucleotides 370–570), which exhibited weak homology to the sequences of related ras genes, were generated and treated with re-hDicer. (B) The secondary structure of H-ras mRNA, as predicted by the mfold program (24). (C) Diced siRNAs were the most effective suppressors of the expression of the endogenous H-ras gene (as indicated by a bar on the far right of the histogram). The results were normalized by reference to levels of actin, as a control. (D) Effects of diced siRNAs targeted to the H-ras gene on the expression of the related K-ras and N-ras genes. K-Ras and N-Ras were detected by western blotting analysis with specific antibodies. Levels were quantitated by densitometry and NIH Image Analysis.

Suppression of the expression of the endogenous H-ras gene. (A) Ten sites (sites 1–10) were chosen as targets for synthetic siRNAs, as detailed in the text. For the preparation of diced siRNAs, long dsRNAs corresponding to the 3′ region of the H-ras gene (nucleotides 370–570), which exhibited weak homology to the sequences of related ras genes, were generated and treated with re-hDicer. (B) The secondary structure of H-ras mRNA, as predicted by the mfold program (24). (C) Diced siRNAs were the most effective suppressors of the expression of the endogenous H-ras gene (as indicated by a bar on the far right of the histogram). The results were normalized by reference to levels of actin, as a control. (D) Effects of diced siRNAs targeted to the H-ras gene on the expression of the related K-ras and N-ras genes. K-Ras and N-Ras were detected by western blotting analysis with specific antibodies. Levels were quantitated by densitometry and NIH Image Analysis.