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. 2003 Feb 1;31(3):911–921. doi: 10.1093/nar/gkg186

Figure 8.

Figure 8

Interaction of recombinant EGR-1 and EGR-4 proteins with NFATp. (A) Recombinant GST-NFATp protein expressed in E.coli was coupled to GST affinity matrix. Various wash and elute fractions were obtained, separated by SDS–PAGE electrophoresis, transferred to nitrocellulose and assayed by western blotting using the indicated antibodies. The individual lanes represent the recombinant protein (lane 1), the material obtained after loading the column (lane 2), the flow through, (lane 3), various wash (lanes 4, 5 and 6) and the elute fraction (lane 7). (B) Interaction of EGR-1 and NFATp. EGR-1 expressed in insect cells (lane 1) was added to an NFATp matrix and wash and elute fractions were assayed with an EGR-1 specific antiserum. (C) Interaction of EGR-4 and NFATp. EGR-4 expressed in insect cells (lane 1) was applied to the NFATp matrix and wash and elute fractions were assayed with an EGR-4 specific antiserum. (D) As control an unrelated recombinant protein (FH SCR 15–20) (lane 1) was assayed for binding and an anti factor H antiserum was used for detection of the individual fractions.