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. 2003 Feb 1;31(3):911–921. doi: 10.1093/nar/gkg186

Figure 9.

Figure 9

Native EGR proteins interact with recombinant NFAT proteins. (A) Detection of Sp1, EGR-1 and EGR-4 proteins in cell extract prepared from stimulated Jurkat T cells by SDS–PAGE and western blotting, using specific antisera for Sp1 (lane 1), EGR-1 (lane 2) and EGR-4 (lane 3). (B) Following application of Jurkat cell extract to a NFATc matrix, the flow through (FT) and elute (EL) fractions were assayed by SDS–PAGE and western blotting with antisera for Sp1 (lanes 1 and 2), EGR-1 (lanes 3 and 4) and EGR-4 (lanes 5 and 6). (C) Following application of cell extract derived from stimulated Jurkat T cells and added to a NFATp matrix, the flow through (FT) and elute (EL) fractions were assayed by SDS–PAGE and western blotting using the indicated antisera for Sp1 (lanes 1 and 2), EGR-1 (lanes 3 and 4) and EGR-4 (lanes 5 and 6). The mobility of the marker proteins is indicated on the left.