Figure 2.

Kinetics of (–) strand strong stop DNA synthesis from the tRNA3^Lys primer. Radiolabeled tRNA₃^Lys was hybridized either to wild type, 2L, 2R or 2LR vRNA and (–) strand strong stop DNA synthesis was initiated by addition of a mixture of the four deoxyribonucleotide triphosphates and RT. The reaction was stopped after 0, 15, 30 and 45 s, 1, 2, 3, 4, 5, 10, 20, 30, 40, 50 and 60 min (lanes 0 to 14, respectively), and the reaction products were analyzed on an 8% denaturing polyacrylamide gel. The products corresponding to extension of the primer by 3, 13, 14, 48, 49 and 181 nt are indicated on the right hand side of the gels. The larger product is the (–) strand strong stop DNA.