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. 2003 Feb 1;31(3):850–859. doi: 10.1093/nar/gkg187

Figure 6.

Figure 6

Kinetics of (–) strand strong stop DNA synthesis from the ORN primer. Radiolabeled ORN was hybridized either to wild type, 2L, 2R or 2LR vRNA and (–) strand strong stop DNA synthesis was initiated by addition of a mixture of the four deoxyribonucleotide triphosphates and RT. The reaction was stopped after 0, 15, 30 and 45 s, 1, 2, 3, 4, 5, 10, 20, 30, 40, 50 and 60 min (lanes 0 to 14, respectively), and the reaction products were analyzed on 15% denaturing polyacrylamide gel. The products corresponding to extension of the primer by 3, 13, 14, 48, 49 and 181 nt are indicated.