Figure 1.

Apc11 Mutants arrest in G2/M as large-budded cells with short mitotic spindles. (A) Yeast strains bearing wild-type (APC11) or temperature-sensitive (apc11–13 and apc11–22) alleles of APC11 were grown to midlog phase at 25°C, shifted to 37°C for 3 h, and analyzed for DNA content by fluorescence-activated cell sorting (FACS). (B) Wild-type and apc11 ts cells incubated at 37°C for 3 h were analyzed by DAPI staining (left panels) or by immunofluorescence staining for mitotic spindles with antitubulin antibody (right panels).