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. 2000 Jul;11(7):2315–2325. doi: 10.1091/mbc.11.7.2315

Figure 4.

Figure 4

Wild-type and mutant versions of Apc11p assemble into the anaphase-promoting complex. W303 cells were transformed with centromeric plasmids expressing wild-type (WT) or mutant (41, 44, 80, 81, and 91, as described in Figure 2) versions of Apc11p bearing 3 HA epitope tags at their C-termini. Apc11pHA3 proteins were immunoprecipitated from cell extracts using monoclonal antibody 12CA5 and separated by SDS-PAGE. Gels were then blotted, cut into sections, and probed with either anti-HA monoclonal antibody (upper panel), anti-Cdc16 polyclonal antibody (middle panel), or anti-Cdc27 polyclonal antibody (lower panel). An arrow indicates immunoprecipitated Apc11pHA3 proteins, and the asterisk indicates the position of immunoglobulin light chain, which was detected in the 12CA5 immunoblot.