EMSA super-shift analysis of nuclear protein binding to GC-box 2 using antibodies to specific transcription factors. The results of EMSA super-shift experiments are shown following the addition of antibodies that are specific for the EGR-1, Sp1, Sp3, Sp4 factors or pair-wise combinations of Sp1, Sp3 and Sp4 to nuclear extracts prior to the addition of radiolabeled GC-box 2 probe. Arrows mark the positions of super-shifted EGR-1 and Sp1 complexes, respectively.