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. 2003 Feb 1;31(3):999–1005. doi: 10.1093/nar/gkg197

Figure 1.

Figure 1

(A) Western blotting analysis of FCP1-stably expressing cell clones. Different H1299 cell clones were assayed for FCP1 expression by using an FCP1-specific antibody. Clone 4, expressing lower amount of exogenous versus endogenous FCP1, was chosen for further analysis. The efficiency of the immunoaffinity of nuclear extracts from cell clone 4 with M2 anti-FLAG beads is shown below (NE, nuclear extract; IP, bound material; PostIP, unbound material). (B) Nuclear extracts from HFFCP1 cells (NE, nuclear extracts; IP, FLAG-affinity material) were assayed in western blots using the indicated antibodies.