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. 2003 Feb 1;31(3):e7. doi: 10.1093/nar/gng007

Figure 4.

Figure 4

Primer extension with deoxynucleotide triphosphates. The positions of unextended (inverted open triangle), singly extended (inverted black triangle) and doubly extended primers (inverted grey triangle) are indicated. All spectra contain one additional equivalent of primer as a post-reaction standard. (A–D) Incorporation of dGTP for the following primer/template/polymerase combinations. (A) P1/LTT/exo– KF. (B) SP1/LTT/exo– KF. (C) SP1/LTT/exo+ KF. (D) SP1/LTC/exo+ KF. (E–H) Incorporation of dATP for the following primer/template/polymerase combinations. (E) P1/LTT/exo– KF. (F) SP1/LTT/exo– KF. (G) SP1/LTT/exo+ KF. (H) SP1/LTT/T4 DNAP. All spectra include a signal from primer added post-reaction as an internal standard.